FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26491993
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG349400.011.60739880-81
TAGGGGG130150.011.06196150-51
CGCGTAT6151.8151513E-710.8054581
AAAAAGG472150.010.11996378-79
CGCGAAT9501.0695658E-99.4933671
CGGGGAT78000.09.4325111
AGGGGGG491950.09.27945482-83
CGCGTAA4850.00222006678.8082781
AAGGGGG347250.08.70031482-83
AAAAGGG347900.08.43406280-81
CGGGAAT59300.08.3247061
TAAAAAA764850.08.18782870-71
CGCGAGT24350.07.99236251
CGGGGTT87550.07.96987341
TATAGGG63500.07.894315748-49
GATCGGA443350.07.53539394-95
GGGGGGT464800.07.52617914-15
AGCGTCG215000.07.46033394-95
GCGTCGT181400.07.37720594-95
CGGGTAT32200.07.37062641
Produced by FastQC (version 0.11.5)