FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27126974
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG379700.012.76501480-81
TAGGGGG139400.012.35606350-51
CGCGAAT8901.8189894E-1211.200421
AAAAAGG518650.010.91701378-79
AAGGGGG377700.010.55805582-83
AAAAGGG383200.010.54035380-81
GGGGGGA560150.09.8625232
AGGGGGG550100.09.5979482-83
TATAGGG72650.09.58148648-49
TAAAAAA834450.09.47816870-71
CGGGGAT75100.09.1650131
GGGAAAA377000.08.83288422-23
CGCGAGT24350.08.7724041
GGGGGGT494000.08.4898814-15
GGGGGAG542850.08.4191653
CGCGTAA6554.5826688E-47.97180031
CGGGTAT31500.07.83606151
AAAAAAG741500.07.744968478-79
TAAGGGG109950.07.713968350-51
GCGTCGT189700.07.667703694-95
Produced by FastQC (version 0.11.5)