FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21797365
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG275700.010.72886680-81
TAGGGGG109000.09.58991950-51
CGCGATT8501.6880222E-89.493591
CGCGAGT19900.09.3027661
AAAAAGG375650.09.16481478-79
AGGGGGG406650.08.55103882-83
CGGGATT41350.08.4948691
CGGGGAT63650.08.4271471
CCGGGAT71350.08.2495121
CTCGGAT59500.08.2171411
CGGGAAT52650.08.1141811
AAAAGGG273800.07.966139378-79
ATCGCGT7854.626019E-57.86008746
TAAAAAA621950.07.68164870-71
CGGGGTT75150.07.64287761
GCGTCGT149200.07.617809394-95
CGGAGAT77350.07.48686551
AAGGGGG272350.07.44119880-81
GATCGGA346900.07.3188594-95
GGGGGGA372450.07.22523262
Produced by FastQC (version 0.11.5)