FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24589518
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253110.10293410387304054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG332200.012.67353180-81
AAAAAGG453150.010.74894378-79
AAAAGGG334500.09.5183778-79
TAGGGGG135250.09.36261950-51
AGGGGGG554200.09.162971582-83
TAAAAAA744600.09.13188170-71
CGGGCAT93450.08.9896291
AAGGGGG334050.08.96174580-81
AAAAAAG673100.08.58854376-77
CGCGTAA5000.00292859658.5431941
CGCGAAT9551.11745976E-78.4487681
CGGGATT43300.08.4401591
CGGGGAT69600.08.3195221
GTCGATT28300.08.2178351
CTCGGAT67000.08.0756571
CCGGAAT55100.07.83858351
CCGGGAT74650.07.75671431
CGAGAAT51250.07.686561
TAGATCG69000.07.565067394-95
GCGTCGT165850.07.53935794-95
Produced by FastQC (version 0.11.5)