FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26863036
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG130500.011.1778850-51
AAAGGGG337700.010.609570580-81
CGGGGAT72750.09.1992691
AAAAAGG465950.09.08696778-79
CGGGTAT32700.08.999351
TATAGGG65250.08.88404848-49
AAGGGGG328350.08.87583482-83
AGGGGGG464900.08.79271482-83
GGGGGGA456700.08.7702572
CCGGGAT84800.08.3958091
AAAAGGG336250.08.34478580-81
TAAAAAA771500.08.04658470-71
CGCGCAT19000.07.99398851
CGGGAAT60150.07.9699011
CGGGCAT99550.07.96237041
AATCGAA40000.07.9486735
GGGGGGT436500.07.89994614-15
CGCGATT8852.5125142E-57.50847861
GTCCGAT27100.07.3560931
GCGTCGT193100.07.298374794-95
Produced by FastQC (version 0.11.5)