FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25303431
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG313050.010.75408780-81
CGGGGAT74550.010.5052011
AAAAAGG432900.09.3798678-79
TAGGGGG123950.08.70196350-51
AGGGGGG472850.08.448647582-83
TAAAAAA714250.08.14902670-71
CGGGGTT91450.08.0967171
CGCGAAT10253.471905E-77.8721461
CGCGGAT37700.07.6799181
GGGGGGA435750.07.6238552
AAGGGGG313450.07.577332582-83
CGGGATT45150.07.56907561
GCGTCGT180900.07.55522394-95
CGAAAAT55950.07.55019241
CCGGGAT79100.07.5006971
CGGGTAT29150.07.49009561
AAAAGGG317400.07.485743578-79
CGGGAAT56500.07.47669461
CGATCGT8309.259328E-57.4331414
CGCGAGT24700.07.3022171
Produced by FastQC (version 0.11.5)