FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25695332
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG316850.010.09328680-81
CGGGAAT54900.08.9047081
AAAAAGG447950.08.50825778-79
GCGGGAT60600.08.4587451
TAGGGGG110400.08.26362850-51
CGGGGAT74550.08.2129061
CCGGGAT80050.07.82649661
TAAAAAA714750.07.78485870-71
GGGGGGA449650.07.7256132
AGGGGGG493200.07.62814482-83
AATCGTG43500.07.52721555
GCGTCGT210100.07.453253394-95
GATCGGA485550.07.41275594-95
AAGGGGG323950.07.383091482-83
CGCCGAT41600.07.18791
CGACGAT30400.07.1818951
ATCGGAA455250.07.124371594-95
AAAAGGG321200.07.108681780-81
CGGCAAT56300.07.0815071
TCGCATT30850.07.07618432
Produced by FastQC (version 0.11.5)