FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25969943
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG292600.010.46678980-81
TAGGGGG116800.09.3161850-51
CGCGAGT23950.09.1161821
CGGGAAT53700.09.0154321
AAAAAGG424050.08.53942878-79
AGGGGGG424150.08.42760582-83
GGGGGGA405700.08.2349112
CCGGTAT31850.08.0472031
CGGGCAT103250.07.9067691
CTCGGTT49900.07.8947471
AAGGGGG301000.07.75664982-83
CGGGGAT74250.07.7348091
CGGGTAT26600.07.6726871
CGGGGTT87000.07.637821
TAAAAAA698000.07.53588270-71
AAAAGGG301500.07.45484780-81
GAAATCG64800.07.2499863
GATCGGA449950.07.234895794-95
ATCGGAA427350.07.21775494-95
AGCGTCG224500.07.1022494-95
Produced by FastQC (version 0.11.5)