FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27105538
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281200.10374263739018942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG325150.010.6681980-81
AAAAGGG324600.010.61352578-79
CGGGGAT76550.09.3007391
AAAAAAG659050.08.017920576-77
AGGGGGG511650.07.9935982-83
AAAGGGG318500.07.877277480-81
GTCGAAT25950.07.86506751
CGGACAT54800.07.79530861
CCGGGAT85400.07.78111031
CGCGTAA6100.0020061587.78111031
GATCGGA534500.07.65335894-95
ATCGGAA503400.07.593549394-95
GCGTCGT230900.07.42979594-95
GCGGGAT67000.07.3676661
CGTCGTG227550.07.288913794-95
CGGGATT45850.07.246531
AGATCGG605300.07.23249594-95
GTCGTGT211200.07.201559594-95
GTCGGTT45600.07.18216851
CGCGTAT7350.00156695737.10357141
Produced by FastQC (version 0.11.5)