FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27302964
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG323550.010.38304780-81
CGGGGAT75050.010.0562561
TAGGGGG124900.09.79585850-51
CGCGAAT11353.6379788E-129.6188221
CGGGTAT30450.08.7295261
AGGGGGG452550.08.61764182-83
CGGGGTT90950.08.6113481
AAAAAGG459800.08.60381778-79
CCGGGAT81900.08.4037681
AAGGGGG326200.08.20581782-83
GGGGGGA439600.08.097182
AAAAGGG326850.08.01032180-81
CTCGTAT24300.07.8134641
GCGTCGT219650.07.79980994-95
GCCGATT27250.07.66436241
CGGGATT46600.07.53764531
TATAGGG63500.07.51998348-49
TAAAAAA769900.07.48605570-71
AGCGTCG254500.07.421697694-95
CGTCGTG211300.07.411778594-95
Produced by FastQC (version 0.11.5)