FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24333449
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT9801.6370905E-1110.1711611
AAAGGGG278200.09.787230580-81
CGGGGAT72000.09.0316141
CTCGTAT21700.08.7493861
TAGGGGG107000.08.74807750-51
CGGGAAT52150.08.7376421
AAAAAGG399400.08.04295678-79
AGGGGGG412350.07.949020482-83
AAAAGGG288200.07.939248678-79
CGGGTAT28150.07.92495441
GCGTCGT193250.07.833456594-95
CGGGGTT86500.07.79201131
GCCGGTT51900.07.77372031
GATCGGA468850.07.687340794-95
CGTCGTG192600.07.6627894-95
AGATCGG527800.07.637940494-95
CGCGGAT35350.07.51927471
GGCGAAT30950.07.5147191
GGGAAAT195900.07.4626591
AGCGTCG225250.07.436903594-95
Produced by FastQC (version 0.11.5)