FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n02_HBLm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n02_HBLm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30656814
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340290.11099979273775806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG343250.09.99536978-79
AAGGGGG354000.09.56383780-81
CGGGGAT94600.09.18261
CGGGTAT32650.08.2871
AAAGGGG343350.07.97834580-81
CGCGGAT45850.07.97181271
CGCGATT9651.1752818E-67.8704281
AGGGGGG553450.07.59998782-83
CGGGAAT70650.07.59227561
GCGTCGT298000.07.556939694-95
GTCGATT32850.07.5140431
CGGGCAT127500.07.48327261
GGGGAAT162900.07.4306151
AAAAAAG725350.07.40629176-77
AGATCGG785650.07.369817794-95
TACCGTA22550.07.3689669
CGTCGTG285150.07.339917794-95
GATCGGA698650.07.319536794-95
AGCGTCG338500.07.31876594-95
CGGGATT54750.07.28284171
Produced by FastQC (version 0.11.5)