FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25498540
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23754079.315854946989122No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT277790.10894349245094033TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC335000.047.285488
CGGAAGA351950.045.3905034
TCGGAAG349200.045.3261763
ATCGGAA353250.045.0881462
GATCGGA350500.044.3389851
TATCTCG68100.034.82251740-41
CTCGTAT100100.034.50063344-45
GAGCACA465850.034.419669
TCTCGTA105600.034.3524942-43
ATGCCGT121550.032.84306348-49
AAGAGCA519850.031.7271167
CGTCTGA249750.031.07112116-17
ACGTCTG252200.030.65508314-15
ACACGTC255250.030.26688812-13
CACGTCT254500.030.24786414-15
GAAGAGC545650.029.668946
CACACGT266100.029.27286712-13
ATCTCGT117250.028.9333342-43
GGAAGAG587800.027.8474145
GCACACG283500.027.48559810-11
Produced by FastQC (version 0.11.5)