FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26470181
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1875780.7086389020158192TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG240050.043.50103844-45
CGTATGC248550.042.49080346-47
TATGCCG249750.042.0008648-49
CTCGTAT237550.041.86937744-45
TCTCGTA249600.038.31615442-43
GTCACGA282550.037.73082428-29
ATGCCGT294200.035.59046648-49
CGAGATT300000.035.44135332-33
TCACGAG303050.035.3979930-31
GTATGCC299300.035.19868546-47
AGTCACG306850.034.9518228-29
ATCTCGT274400.034.77528442-43
CCGTCTT301250.034.756952-53
GCCGTCT307400.033.9460750-51
GAGCACA637950.033.912669
CACGAGA315450.033.78820830-31
ACGAGAT321450.033.35713632-33
AGAGCAC653800.032.981558
TGCCGTC322400.032.6466150-51
TCGGAAG675950.031.6057263
Produced by FastQC (version 0.11.5)