FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27507391
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2414530.8777749950913193TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG302700.043.84160644-45
CGTATGC307350.043.52535246-47
TATGCCG314950.042.4366948-49
CTCGTAT297500.042.34877844-45
TCTCGTA308900.039.38710442-43
GTCACGA350450.038.54570428-29
GAGCACA735600.037.1845789
GTATGCC360500.037.0818846-47
CGAGATT364050.037.07315432-33
ATGCCGT359600.037.06844748-49
TCACGAG368300.036.87096430-31
CCGTCTT362900.036.65254252-53
ATCTCGT330550.036.62056742-43
AGTCACG374650.036.1383128-29
AGAGCAC755050.036.0822268
GCCGTCT369650.035.964450-51
CACGAGA381800.035.51127230-31
TGCCGTC384500.034.82245350-51
TCGGAAG780500.034.6384283
ACGAGAT391650.034.58184432-33
Produced by FastQC (version 0.11.5)