FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26491993
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2461210.9290392006369623TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG312350.044.0151644-45
CGTATGC317750.043.5737446-47
TATGCCG321250.042.93648-49
CTCGTAT304250.042.91551244-45
GAGCACA691650.041.1888439
AGAGCAC700500.040.5262378
TCTCGTA314100.040.163542-43
GTCACGA352650.039.8200428-29
ATCGGAA714950.039.35552
TCGGAAG718900.039.2974553
CGGAAGA729950.038.6829764
CGAGATT368000.038.17878732-33
TCACGAG372750.037.95313630-31
GATCGGA739500.037.8724441
ATGCCGT363800.037.7510248-49
AGTCACG375600.037.595628-29
CCGTCTT367400.037.4126552-53
GTATGCC369650.037.21813646-47
ATCTCGT339950.037.032642-43
CACGAGA387250.036.26220330-31
Produced by FastQC (version 0.11.5)