FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27126974
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3063361.129267127251274TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA783700.045.380789
AGAGCAC797600.044.5065468
TCGTATG388150.044.43884744-45
CGTATGC392650.044.24922646-47
ATCGGAA801300.043.963752
TATGCCG396750.043.6777448-49
CTCGTAT376450.043.60565644-45
TCGGAAG813750.043.4074553
CGGAAGA817000.043.2173424
GATCGGA823950.042.5057371
GTCACGA427300.041.0284128-29
TCTCGTA389100.040.94312342-43
TCACGAG442200.039.83392330-31
CGAGATT444450.039.46687732-33
AAGAGCA902600.039.3395237
AGTCACG450400.039.2194428-29
ATGCCGT444900.038.84922848-49
GTATGCC448600.038.5927646-47
CCGTCTT448400.038.43455552-53
ATCTCGT412750.038.4302842-43
Produced by FastQC (version 0.11.5)