FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21797365
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2052950.9418340244336872TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG245400.043.6073844-45
CGTATGC248600.043.23657646-47
CTCGTAT241600.042.8679344-45
TATGCCG251200.042.5713848-49
TATCTCG253850.040.63081440-41
TCTCGTA256700.040.1613542-43
GAGCACA555100.039.5557029
AGAGCAC557200.039.4321988
ATCGGAA555150.039.125622
TCGGAAG564700.038.606223
CGGAAGA572550.038.118214
GATCGGA574700.037.6698841
ATCTCGT278400.037.19303542-43
ATGCCGT288650.037.0316348-49
ACCGCTC295150.036.5703432-33
GTATGCC293550.036.5188746-47
CCGTCTT292300.036.45488452-53
GCCGTCT296300.035.91531850-51
CGCTCAT305050.035.35997834-35
TGCCGTC309500.034.57538250-51
Produced by FastQC (version 0.11.5)