FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24589518
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2958221.2030410681494448TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA693600.045.7363329
ATCGGAA698950.045.1360172
TCGGAAG708200.044.6934853
AGAGCAC709550.044.6749348
TCGTATG348000.044.5701444-45
CGTATGC352850.044.1593446-47
CGGAAGA718350.044.055194
GATCGGA719500.043.7007521
TATGCCG357000.043.49903548-49
CTCGTAT345900.043.48816344-45
TATCTCG355300.042.08348540-41
TCTCGTA363050.041.13934742-43
AAGAGCA807300.039.3182877
ATGCCGT397300.038.93728648-49
ATCTCGT387150.038.8176742-43
GTATGCC403200.038.5447746-47
CCGTCTT405100.038.13418252-53
ACCGCTC409750.038.06802732-33
GCCGTCT410050.037.58761650-51
CGCTCAT420650.037.12687734-35
Produced by FastQC (version 0.11.5)