FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26863036
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2516190.9366737251887686TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG308450.043.532444-45
CGTATGC312950.043.2246846-47
CTCGTAT307450.042.7161644-45
TATGCCG316650.042.63643348-49
TATCTCG317900.040.8641140-41
TCTCGTA326700.039.87254342-43
GAGCACA705100.039.2037249
AGAGCAC713100.038.7640538
ATCGGAA715150.038.175392
TCGGAAG722850.037.9395073
CGGAAGA726900.037.7083784
ATCTCGT349800.037.43634442-43
ATGCCGT359550.037.370948-49
GATCGGA737100.036.8624841
CCGTCTT365150.036.7263352-53
ACCGCTC372650.036.3064932-33
GTATGCC371800.036.27431546-47
GCCGTCT373950.035.7479550-51
TGCCGTC385200.035.06149750-51
CGCTCAT390550.034.8612734-35
Produced by FastQC (version 0.11.5)