FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25303431
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2418770.9559059401865305TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG289400.043.61587544-45
CGTATGC295200.043.20901546-47
TATGCCG296900.042.79329748-49
CTCGTAT290350.042.42621644-45
TATCTCG299850.040.59947240-41
TCTCGTA304250.040.09803842-43
GAGCACA654500.039.622179
AGAGCAC666550.038.9130028
ATCGGAA660700.038.877082
TCGGAAG671100.038.366313
CGGAAGA680950.037.860164
GATCGGA683400.037.538831
ATGCCGT340200.037.24193648-49
ATCTCGT330900.037.14853342-43
CCGTCTT344100.036.75067552-53
GTATGCC346000.036.70030246-47
ACCGCTC348000.036.69476332-33
GCCGTCT351150.035.87793450-51
CGCTCAT362600.035.2895134-35
TGCCGTC365450.034.7859650-51
Produced by FastQC (version 0.11.5)