FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25695332
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2329620.9066316014130503TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG282550.043.46312344-45
CGTATGC286100.043.03140346-47
TATGCCG285250.042.9011648-49
CTCGTAT278300.042.72736744-45
TATCTCG286500.041.2142540-41
TCTCGTA298950.039.53764742-43
ATGCCGT327600.037.3189348-49
GAGCACA677250.037.098949
GTATGCC333750.036.8450546-47
AGAGCAC688750.036.334698
ACCGCTC344250.035.90073832-33
CCGTCTT341150.035.66960552-53
ATCTCGT334000.035.57343342-43
GCCGTCT349200.034.8815350-51
CGCTCAT357800.034.74025734-35
ATCGGAA719050.034.6722832
TGCCGTC355300.034.5166150-51
TCGGAAG725700.034.49143
CACCGCT362050.034.10909730-31
CGGAAGA733750.034.1063884
Produced by FastQC (version 0.11.5)