FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25969943
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2181000.8398170146157039TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG261750.043.45150844-45
CGTATGC266700.042.67118546-47
TATGCCG266100.042.6420848-49
CTCGTAT262400.042.10392844-45
TATCTCG270300.040.61028740-41
TCTCGTA283250.038.7785442-43
ATGCCGT309100.036.74072348-49
GAGCACA645950.036.176269
GTATGCC317250.035.9543846-47
AGAGCAC651300.035.660328
CCGTCTT319900.035.33646852-53
ACCGCTC323600.035.2120932-33
ATCTCGT316000.034.90236342-43
GCCGTCT326800.034.5690150-51
CGCTCAT334000.034.36485734-35
ATCGGAA670850.034.289012
TCGGAAG680700.033.8901943
TGCCGTC338450.033.56854250-51
CGGAAGA687850.033.551534
GATCGGA693000.033.0160751
Produced by FastQC (version 0.11.5)