FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27105538
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2601560.9597891028763199TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG313250.043.7227144-45
CGTATGC317700.043.3188846-47
TATGCCG318150.043.21254348-49
CTCGTAT312800.042.46452744-45
TATCTCG321800.040.85641540-41
TCTCGTA333550.039.4741242-43
GTATGCC366950.037.51133346-47
GAGCACA755550.037.4221279
ATGCCGT365950.037.3799748-49
ACCGCTC375800.036.7671832-33
AGAGCAC765500.036.7620548
CCGTCTT373950.036.2811752-53
GCCGTCT380400.035.63549850-51
ATCTCGT372050.035.61273242-43
CGCTCAT393500.035.21595834-35
TGCCGTC392550.034.85317250-51
CGGAAGA813600.034.3668484
ATCGGAA809600.034.3610232
TCGGAAG813100.034.341313
CACCGCT400500.034.33300830-31
Produced by FastQC (version 0.11.5)