FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27302964
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2418600.8858378892489475TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG285950.043.58614344-45
CGTATGC290250.043.16082846-47
TATGCCG293500.042.58564448-49
CTCGTAT287400.042.3581144-45
TATCTCG299200.040.30660640-41
TCTCGTA308850.039.1010242-43
ATGCCGT337400.036.8476348-49
GTATGCC342250.036.54764646-47
GAGCACA713050.035.969359
CCGTCTT346250.035.898552-53
AGAGCAC718050.035.6594168
ACCGCTC352900.035.5730132-33
ATCTCGT348050.034.91551642-43
GCCGTCT354900.034.8565450-51
CGCTCAT366550.034.31324834-35
ATCGGAA745200.034.0677342
TCGGAAG747700.034.0424463
TGCCGTC367450.033.9245750-51
CGGAAGA754100.033.866844
CACCGCT377300.033.17750530-31
Produced by FastQC (version 0.11.5)