FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24333449
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT283990.11670766441699242TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG46000.037.79236640-41
AATCGGG146800.036.43285440-41
GAATCGG154350.035.4508540-41
CACTCCG287400.034.7549330-31
AGAATCG216350.034.23800338-39
GAGCACA606100.033.6568459
AGAGCAC615950.033.0878458
ACTCCGG303400.033.03978732-33
ATCGCGT24050.032.38986642-43
CGGAGAA316950.031.37298836-37
ATCGGAA654300.030.8807332
TCGGAAG660550.030.7030893
CCGGAGA325550.030.64590534-35
CGGAAGA660300.030.6283284
TCCGGAG331500.030.41106834-35
CGCGTAT14050.029.9185944-45
CTCCGGA336850.029.82936132-33
GATCGGA683750.029.4515551
GTCACTC347250.029.06562828-29
AAGAGCA725850.028.1236887
Produced by FastQC (version 0.11.5)