FastQCFastQC Report
Thu 7 Feb 2019
HTVCNBGX9_n01_HBLm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVCNBGX9_n01_HBLm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30656814
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT373170.121724977683591TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG57000.039.33143240-41
AATCGGG185600.036.25026740-41
GAATCGG196750.035.0046640-41
AGAATCG274300.034.0779138-39
CACTCCG372100.034.0622930-31
ACTCCGG391150.032.4521332-33
ATCGCGT33050.032.4076142-43
GAGCACA826300.031.0969149
CGGAGAA409550.030.70447736-37
AGAGCAC842900.030.490138
CCGGAGA422950.029.86635434-35
CGCGTAT20050.029.4934244-45
TCCGGAG431400.029.47953234-35
CTCCGGA436250.029.26050432-33
GTCACTC442700.028.89326928-29
CGGAAGA926200.027.5431634
TCGGAAG930100.027.4378853
ATCGGAA931250.027.312422
ATCGGGT92800.026.46150242-43
GATCGGA972400.026.0124861
Produced by FastQC (version 0.11.5)