FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8706138
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2004222.3020769944147452No Hit
GGGGGGCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG479060.5502554634442964No Hit
GGGGGGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434550.4991306133672589No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272590.31310094096831453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGT75250.057.374793
CTTATAG32650.055.4012835
AGCGGGG100750.054.8689967
CGTATAG28350.054.1781235
GGGGGCG433450.053.266492
ATGGGGG108450.052.7476587
GTATAGG53900.052.1241846
CTATAGG33650.052.0912936
GGGCGTG81550.052.041744
TATAGGG58850.051.723487
GAGCGGG72450.051.72066
GGGGGGC448100.051.7079931
CGCGGGG120850.050.8674627
TATAGGC40100.050.8673557
GCGCGGG98850.050.2601936
TTATAGT70200.048.9924166
GGTATAG51050.048.934485
GGGCGGG332950.048.6433144
AGTATAG44150.048.4990465
CGGATAG18000.048.3993265
Produced by FastQC (version 0.11.5)