FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH97_MTS30mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH97_MTS30mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000694
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG76950.057.4879536
TATAGGG74450.054.153467
CTTATAG34600.054.1148035
TTATAGT87800.053.9712646
GTTATAG81150.053.908835
CGGATAG17050.052.9582635
GTATAGG71800.052.691396
GCGATAG17200.052.2929465
TATAGTG97300.051.902937
CGTATAG29500.051.725295
CGTATAA25700.051.6112675
GGTATAG63900.051.5379565
ATAGGCG17950.051.2778368
CTATAGG37750.051.268036
ATAGGTG60200.051.043038
TATAGGT87600.050.9782567
ATAGGGC22650.050.680878
TTATAAG59050.049.9627236
TATAGGC43850.049.8825727
GGTTATA75850.049.647244
Produced by FastQC (version 0.11.5)