FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH96_MTS30mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH96_MTS30mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4328165
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG11800.060.209045
GTATAGG45250.059.2458426
TTATAGG53100.059.121526
CTTATAG26700.058.068445
CGTATAG23100.057.2700425
CTATAGG32100.056.8041956
TCTACGG12150.056.7443437
TATAGGG55000.056.5681157
CGTATAA18450.056.3388255
TATAGGT57900.055.7294777
GGTATAG39350.055.6771855
CATATAG34200.054.237225
TATAGGC33900.054.1991237
GTTATAG49050.053.7283675
GCGATAG13700.053.647125
CCTATAC7600.053.4185455
TTTATAG54200.053.3369875
TTATAGT54750.053.312576
TTATAAG39950.053.1764836
CGATAGG12400.053.062036