FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH95_MTS30mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH95_MTS30mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5530242
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG68300.058.159296
GTATAGG56150.056.657586
CTTATAG33700.055.8722655
CGTATAG28400.055.701135
CGGATAG16300.055.3956955
TATAGGG64600.055.260097
CGTATAA25150.055.1062855
GGTATAG49550.054.7396435
CTATAGG31700.054.6499566
TATAGGT73950.053.763077
CGATAGG15000.053.663696
TATAGGC37050.053.4653137
CCTATAC11500.052.953585
TTATAAG53100.052.92556
TTTATAG70800.052.595955
GCGATAG16600.052.286255
TTATAGT72850.052.076726
TATAGTG74150.052.0132947
TATAAGG54600.051.9201937
TCTACGG12300.051.50127
Produced by FastQC (version 0.11.5)