FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH91_MTS20mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH91_MTS20mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255470
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG21300.057.507026
CGGATAG6050.057.2680325
TTATAGG27350.056.3025136
CTATAGG12900.055.6155936
TATAGGT28800.055.047577
CTTATAG13100.055.0336535
GGTATAG21000.054.828845
GCGATAG7050.054.6054535
CGATAGG5850.053.8417366
CGTATAA10250.052.9224855
TATAGGG25700.052.8361327
CGTATAG10800.052.4956935
CGATAGT6850.052.1125146
ATAGGTG17150.051.832488
GATAGGT13550.051.3987
TTATAAG22150.050.560196
TATAGTG30450.050.225777
TTATAGT31550.049.805746
AGTATAG16150.049.1910135
TTTATAG28900.049.1655165
Produced by FastQC (version 0.11.5)