FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH90_MTS20mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH90_MTS20mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379973
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA12550.059.1197785
CGATAGT7200.058.815736
TTATAGG26200.057.7062876
CTTATAG13700.057.4781275
CGTATAG12850.057.4671945
GTATAGG22350.056.6854676
CTATAGG15750.056.6630946
ATAGGGC8050.055.6498348
TATAGGG24800.055.6013347
GCGATAG8000.054.684055
CGATAGG6650.053.680836
TATAGGC16550.053.501167
TTATAGT32400.053.0367776
TATAGGT31050.052.8630147
CGGATAG7300.052.7364045
ATAGGCG6650.052.629378
GGTATAG22750.052.4582335
ACGATAG5350.052.3331535
TCTACGG5300.052.1665237
GCGTATA15350.051.9836964
Produced by FastQC (version 0.11.5)