FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH89_MTS20mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH89_MTS20mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5290922
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG31300.059.7091645
TTATAGG62500.057.676826
CTATAGG31700.056.6372536
GTATAGG50600.055.8862426
CGTATAG26850.055.2669645
CGGATAG14300.055.066895
TATAGGG62400.054.6849987
CGTATAA25700.054.1993375
TTATAGT67850.053.851126
TATAGGC36750.053.7118267
TATAGGT66600.052.60277
CGATAGT17850.052.3500486
GCGATAG16600.052.2862665
GATAGGT29100.051.9563837
CCTATAC11050.051.9428335
GGTATAG47150.051.8847275
TATAGTG71900.051.8404667
ATAGGGC19400.051.4151738
CGATAGG14100.051.3801426
TTTATAG66600.050.6578675
Produced by FastQC (version 0.11.5)