FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH85_MTS15mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH85_MTS15mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688121
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG28950.060.4466065
CGTATAG23800.060.2916835
TTATAGG56200.059.84576
CGGATAG13950.059.4599655
GTATAGG51100.058.2846956
CTATAGG31600.057.0382236
TATAGGT68150.056.7976727
TATAGGG54250.056.6416667
CCTATAC9650.055.852675
TTATAGT60750.055.651486
CGTATAA23450.055.0724035
CGATAGT14250.055.014746
TATAGGC35500.054.517767
GGTATAG45450.054.4422455
GTTATAG55450.054.280955
TCTACGG9950.053.8157737
TATAGTG65300.053.809857
CGTTATA32700.053.7287144
AGTATAG36950.053.2319535
GATAGGT26000.053.1697967
Produced by FastQC (version 0.11.5)