FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH84_MTS15mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH84_MTS15mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10938679
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG129150.059.103026
CGGATAG31750.058.643025
CGTATAG60100.058.117185
CTATAGG69550.057.8190966
GTATAGG112200.057.6444556
CGTATAA55300.057.529055
CTTATAG65350.057.411335
TATAGGG125600.056.6763427
GCGATAG31200.055.5263335
GGTATAG104150.055.043125
TATAGGT144600.054.5055277
TTATAGT153000.054.2361156
TCTACGG24550.054.171717
CGATAGT33700.053.8996436
TATAGTG154600.053.243717
TATAGGC79600.052.8044367
GCGTATA66650.052.4582444
CGATAGG30400.052.0371256
GATAGGT63850.051.9622467
GTTATAG139900.051.8096665
Produced by FastQC (version 0.11.5)