FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH83_MTS15mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH83_MTS15mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622706
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG64900.058.9951366
GTATAGG54600.058.906946
CGGATAG17250.058.025725
CGTATAG28800.057.965495
CTTATAG35400.056.945955
CGTATAA26100.056.4527635
CTATAGG34350.056.445156
TATAGGG59900.055.4466487
GCGATAG17050.055.42215
TATAGGT76000.055.2590687
GGTATAG51700.054.6971175
TTATAGT77000.053.67886
AGTATAG41950.052.8933265
TATAGGC36150.052.6655357
ATAGGGC19650.052.3625378
TATAGTG79950.052.310077
CGATAGT17700.052.200456
CGTTATA40650.051.9158974
CCTATAC12900.051.818795
GTTATAG72350.051.3723685
Produced by FastQC (version 0.11.5)