FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH79_MTS10mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH79_MTS10mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688462
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG12600.058.606465
TTATAGG62250.057.8508266
CTTATAG31000.057.5760735
GTATAGG48300.057.5316666
CGTATAA19950.056.4867445
CTATAGG32500.055.887876
GGTATAG41250.055.741035
CGTATAG23200.055.3619845
TATAGGG58650.054.5407377
TTATAAG44100.054.2814036
TATAGGT69000.053.9678847
CGATAGG12050.052.858886
TTATAGT61700.052.1838656
GCGATAG13550.052.1729775
TATAGGC33850.052.1092347
TATAAGG44700.050.9701967
CCTATAC8550.050.7562035
ATAGGCG13250.050.7140358
TTTATAG58850.050.3103645
GATAGGT28700.050.2409827
Produced by FastQC (version 0.11.5)