FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH78_MTS10mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH78_MTS10mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10582374
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG62800.059.406525
TTATAGG128500.058.447126
CGGATAG30900.057.989325
CGTATAG54400.057.7072075
GTATAGG106000.057.1515126
CTATAGG69300.056.9150626
CGTATAA51850.056.495425
TATAGGT145200.055.3159757
TATAGGG125000.054.399797
CCTATAC24800.054.0484965
GGTATAG96400.053.657935
TATAGGC76950.053.5306247
TTATAGT137200.053.3889856
GCGATAG29600.052.850895
CGATAGT33050.052.7344476
TCTACGG22800.052.6494457
TATAGTG143750.051.92997
ATTATAG116400.051.6543275
ATAGGGC45200.051.6442388
CGATAGG31800.051.175536
Produced by FastQC (version 0.11.5)