FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH77_MTS10mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH77_MTS10mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642872
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG7700.059.99765
GTATAGG16250.059.874536
TTATAGG22700.059.3588566
CTATAGG10800.058.655066
CTTATAG9900.057.9774785
TATAGGG20300.057.7563067
CGTATAA7350.057.616755
CGCTAAC1400.057.49776
GCGTATA8650.057.0497444
AGTATAG13350.056.626955
TATAAGG14900.055.90387
ATTATAG18400.055.5412565
CGATAGG5000.055.2977876
ATAGTCC3950.054.9345138
TATAGGC13150.054.5605557
GGTATAG14850.054.442275
TATAGGT25400.054.426967
TTATAAG15100.054.236246
GCGATAG4600.054.0195775
CATATAG15250.053.932275
Produced by FastQC (version 0.11.5)