FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH73_MTS5mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH73_MTS5mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001796
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG12700.060.3536835
TTATAGG48500.059.968436
CGTATAG20300.058.7924735
CTTATAG24650.058.7823335
CGTATAA19250.057.635755
GTATAGG38750.057.5348666
CTATAGG29450.057.282926
TATAGGG46700.056.5076187
GGTATAG35400.055.7621155
TATAGGT52150.054.897377
CGATAGT12050.054.8956766
TCTACGG9900.054.44257
GCGTATA23000.054.3255044
TATAGGC30100.054.0678337
GCGATAG11050.053.845585
TTATAAG36150.053.8306776
GATAGGT24100.053.7325177
CTATAAC15750.053.554986
CCTATAC9350.053.5288435
TTATAGT49800.053.2725226
Produced by FastQC (version 0.11.5)