FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH72_MTS5mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH72_MTS5mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793383
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG47300.059.4913146
CTTATAG23350.058.7567565
GTATAGG38800.057.4600946
CGGATAG11400.057.1040275
CGTATAG20350.057.09955
TATAGGG47050.057.0543377
CTATAGG27100.056.1795966
CGTATAA19050.056.035535
TTATAAG34350.055.7338756
GCGATAG10350.055.119585
GGTATAG34200.054.9549525
TCTACGG9550.054.605437
TATAGGT53450.054.2170077
TATAGGC28200.053.6151547
CATATAG31350.053.587365
TTATAGT48250.053.3874476
GATAGGT24200.052.787427
GCGATAA10500.052.6655275
GCGTATA21000.052.6655274
GTTATAA33550.052.5771035
Produced by FastQC (version 0.11.5)