FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH71_MTS5mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH71_MTS5mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4492970
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG56200.059.3482366
GTATAGG45650.057.8838546
CGTATAA17950.057.323695
CTTATAG28900.057.039325
CGGATAG13900.056.4006965
CTATAGG32400.055.7385866
TATAGGG55900.055.7204977
CGTATAG24450.054.9672055
CCTATAC8350.054.4889145
TATAGGC34600.054.215757
AGTATAG33250.053.155845
CGATAGT13700.053.136636
GGTATAG44250.053.0713965
CGATAGG13550.052.949986
GCGATAG11900.052.939135
TTATAGT61200.052.8982856
TATAGGT66250.052.720737
TTATAAG43650.052.4378366
ATAGGGC19050.052.17538
GATAGGT30100.051.9737
Produced by FastQC (version 0.11.5)