FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH61_MTS120mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH61_MTS120mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152932
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG19550.062.1213045
CGGATAG9750.059.9473045
TTATAGG38650.059.8562856
GTATAGG31350.058.7227026
CGTATAA16850.057.7434465
CTATAGG20800.057.546726
GCGATAG8650.057.455295
TATAGGG35350.056.0365077
GGTATAG27050.055.5070085
TATAGGC24300.055.0175327
CGTATAG17750.055.012795
ATAGGCG9200.054.3991668
GCGTATA19050.054.0144734
TATAGGT41900.053.87537
AGTATAG22900.053.797865
TATAGTG40450.053.5569887
TTATAGT37600.053.429596
ATAGGGC11700.053.2449158
CGATAGT10100.052.6720286
TTTATAG38800.052.5889665
Produced by FastQC (version 0.11.5)