FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH60_MTS120mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH60_MTS120mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621801
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG33300.062.1123476
CGGATAG8500.059.2895665
GTATAGG26200.058.907696
TATAGGG29250.058.747627
CTATAGG18250.058.4886556
CGTATAG15300.058.328855
CTTATAG16600.057.7666475
CGTATAA13450.056.984475
TATAGGT36200.056.459757
GGTATAG25750.055.316145
CGCGATA5200.055.188074
TTATAGT33750.054.9587446
GTTATAG32850.054.6533365
CCTATAC6150.054.6299485
TATAGGC22050.054.1228267
ATAGGGC10950.054.0141148
ATTATAG28750.053.9262965
GCGATAG8400.053.7458725
GATAGGT14500.052.8580067
TATAGTG34650.052.5212177
Produced by FastQC (version 0.11.5)