FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH5_MTS0mS5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH5_MTS0mS5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813039
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG35000.058.997446
GTATAGG29050.057.587866
CGTATAG13750.057.5247735
CTTATAG17700.057.342145
CGGATAG8500.057.232815
CTATAGG19700.057.205646
CGTATAA12800.057.1459545
TATAGGG32500.055.5648427
TTATAGT39150.055.1572466
TATAGGT39200.054.5492637
ATTATAG30950.053.9394955
GGTATAG26400.053.823425
AGTATAG22400.052.966455
TATAGGC21450.052.5366447
GTATAAG22200.052.4977236
GCGTATA16000.052.497724
GATAGGT17600.052.4958577
CGGGATA9150.052.4020964
GTTATAG34800.052.196015
CGATAGG7800.052.0490236
Produced by FastQC (version 0.11.5)