FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH59_MTS120mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH59_MTS120mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5456798
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG67850.060.3503236
CTTATAG32800.060.2862435
GTATAGG62550.059.756836
CGGATAG17000.059.2907835
CGTATAA26350.059.237655
CTATAGG38500.058.633066
GGTATAG54950.058.531745
GCGATAG15850.057.410015
TATAGGG69750.057.400737
TTATAGT68150.056.3871426
CGTATAG31450.056.308445
TATAGGT84100.056.0124667
CCTATAC11550.055.754445
GTTATAG67650.055.665755
TATAGGC43800.055.372587
AGTATAG43100.055.217315
TTATAAG50100.054.9070856
ATAGGCG18250.054.6539578
TATAGTG73250.054.6102567
CGATAGG17100.054.441386
Produced by FastQC (version 0.11.5)