FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH55_MTS90mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH55_MTS90mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5804286
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG71250.060.9086886
CTTATAG35600.059.0835955
GTATAGG61400.057.6838956
CGTATAG28100.056.7938085
CTATAGG39800.056.4539576
CGTATAA28950.056.2143025
CGGATAG17800.056.0360225
TATAGGC43350.056.0289967
TATAGGG68100.055.451957
TATAGGT84450.054.7037057
GTTATAG72000.054.3926285
GGTATAG54150.054.096545
TTATAGT78550.054.0006376
GCGATAG18650.053.48215
CGATAGT20750.053.4667246
GATAGGT36500.052.6407367
TATAGTG81900.052.4328087
ATAGGGC23450.052.384978
TTTATAG71450.052.36225
AGTATAG46250.052.0617945
Produced by FastQC (version 0.11.5)