FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH54_MTS90mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH54_MTS90mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945478
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG48200.060.192656
GTATAGG45700.059.5798456
CTTATAG27000.059.2363625
CTATAGG27400.057.9884226
CGTATAG22550.057.5788965
TATAGGG48750.057.3605967
GGTATAG38700.057.062975
CGGATAG10700.056.9116825
CGTATAA19450.056.679625
TTATAGT52500.055.395916
TATAGGC31250.055.1006287
GCGATAG11900.054.9959375
TCTACGG9150.054.6961067
TATAGGT59450.054.3364567
GTTATAG48750.053.69865
AGTATAG32150.053.2309045
CGATAGG11600.053.0995256
GATAGGT24000.052.934267
ATTATAG41450.052.854965
CGATAGT12850.052.564216
Produced by FastQC (version 0.11.5)