FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH53_MTS90mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH53_MTS90mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4137517
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG25100.058.8418275
CGGATAG12400.058.4246445
TTATAGG48050.058.124896
GTATAGG39900.056.5769426
TATAGGG45800.055.7827037
CTATAGG25850.055.7813956
CGTATAG21200.055.4690635
CGTATAA20400.054.8993575
GGTATAG37550.054.524715
TATAGGT53250.054.4192927
TTATAGT53500.054.166316
TATAGGC28250.053.89067
AGTATAG30350.052.353365
TATAGTG58250.052.0913167
GTTATAG51500.052.0557825
TTTATAG50300.051.6973465
CGATAGT14150.051.6942836
GATAGGT26050.051.5900157
CGATAGG12300.051.5022436
TATAGTC30900.050.9678427
Produced by FastQC (version 0.11.5)